Medicinal plant material carry a great no. of bacteria and moulds, often originating in the soil, while a large range of bacteria & fungi from naturally occurring micro flora of herbs, aerobic spore forming bacteria frequently predominate. The Total microbial load in Aq. leaf extract of Tylophora indica was found to 103 CFU g/ml in 1:1 dilution, 101 CFU g/ml in 1:10 dilution and 100 CFU g/ml in 1:100 respectively. The leaves of plant Tylophora indica in aqueous and methanolic extract were investigated for anti-microbial activity. The bacterial strains used in the study were Escherchia coli, Group A Streptococcus (GAS), and Group B streptococcus (GBS). It is found that E. coli was not inhibited even at higher concentration of either aqueous or methanolic extract of T. indica. However, Antibacterial activity is found to be exhibited against GAS and GBS by aqueous and methanolic extract of Tylophora indica.
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